Genetic manipulation of glycine decarboxylation

Genetic manipulation of glycine decarboxylation.

The glycine-serine interconversion, catalysed by glycine decarboxylase and serine hydroxymethyltransferase, is an important reaction of primary metabolism in all organisms including plants, by providing one-carbon units for many biosynthetic reactions. In plants, in addition, it is an integral part of the photorespiratory metabolic pathway and produces large amounts of photorespiratory CO(2) wi...

Mechanism of decarboxylation of glycine and glycolate by isolated soybean cells.

Isolated soybean leaf mesophyll cells decarboxylated exogenously added [1-(14)C]glycolate and [1-(14)C]glycine in the dark. The rate of CO(2) release from glycine was inhibited over 90% by isonicotinic acid hydrazide and about 80% by KCN, two inhibitors of the glycine to serine plus CO(2) reaction. The release of CO(2) from glycolate was inhibited by less than 50% under the same conditions. Thi...

Endophytic Fungi, Characteristics and Their Potential for Genetic Manipulation

Genetic Manipulation of Bacteria

Role of Glycine and Glyoxylate Decarboxylation in Photorespiratory CO(2) Release.

Mechanically isolated soybean leaf cells metabolized added glycolate by two mechanisms, the direct oxidation of glyoxylate and the decarboxylation of glycine. The rate of glyoxylate oxidation was dependent on the cellular glyoxylate concentration and was linear between 0.58 and 2.66 micromoles glyoxylate per milligram chlorophyll. The rate extrapolated to zero at a concentration of zero. The co...